The animal born at Dvur Kralove zoo in the country’s north died from complications of a ruptured cyst, the zoo said in a statement on Tuesday, zookeepers said.
“Her death is a symbol of the catastrophic decline of rhinos due to a senseless human greed. Her species is on the very brink of extinction,” said zoo director Premysl Rabas.
The four remaining rhinos include Nola, an elderly female living at a zoo in San Diego, and Sudan, an elderly male living with two females, Najin and her daughter Fatu on the Ol Pejeta reserve in Kenya.
The Ol Pejeta rhinos were shipped from the Czech zoo in 2009 in the hope that the natural environment would encourage breeding. That hope has faded.
If it is possible at all, any IVF attempt will now almost certainly require the implantation of a Northern White Rhino embryo in the closely related Southern White Rhino, which are more numerous.
Dvur Kralove is the world’s only zoo to have succeeded at the captive breeding of the rare rhino.